Hypoxia Workstation with TRUE pO2 control – control the O2 available to your respiring cells!
Lightweight cover & ergonomic design to suit any environment
HypoxyLab’s lightweight and durable cover can be easily removed. This not only allows for easy loading of media and consumables but makes routine cleaning and disinfecting of the workstation a practical reality. Its ergonomic design ensures natural, relaxed operation, with an angled vision panel combined with adjustable LED illumination that provide excellent visibility.
True physiological oxygen (physoxia)
As every scientist knows, it’s the partial pressure of oxygen that reflects the oxygen that cells actually ‘see’. The partial pressure of oxygen varies not only with oxygen concentration in the atmosphere, but also with altitude and prevailing weather conditions. For this reason we believe it is insufficient to express hypoxia in terms of a barometrically uncompensated percent oxygen concentration alone. HypoxyLab is therefore the world’s first and only hypoxia workstation that directly regulates its environment using the partial pressure of oxygen, expressed in absolute units of mmHg or kPa, making it insensitive to meteorological conditions or the altitude of the laboratory. This scientifically rigorous approach provides researchers the ability to replicate physiological oxygen in vitro, at the highest levels of accuracy and reproducibility.
See our related article, “The importance of oxygen partial pressure in cell culture“
Hypoxia chamber with proprietary gas control technology
HypoxyLab uses purpose-designed gas control hardware and an advanced Model Predictive Control (MPC) gas control algorithm, developed in-house, to create and maintain a fully humidified, highly stable oxygen-controlled environment for tissue culture. It responds rapidly to single or multiple setpoint changes, with minimal or zero overshoot characteristics, all while consuming surprisingly little nitrogen gas.
Hypoxia chamber offering in vitro dissolved oxygen from media & cultures
Dissolved oxygen can optionally be sampled directly and continuously from cell cultures (e.g. from the cell monolayer itself) and/or from media being conditioned within the HypoxyLab, via a miniature optical sensor that can be fed into the chamber through a dedicated, sealable side-gland. Oxygen readings (in mmHg) can be read from the OxyLite™ monitor or can be displayed and recorded by the HypoxyLab itself. OxyLite™ monitor required.
Easy-entry hatch system
HypoxyLab’s ‘letterbox’ hatch entry system affords quick and easy transfer of cell plates and accessories into or out of the chamber without the need for an air lock or isolation hatch. HypoxyLab senses when items are passed through the hatch, temporarily generating a slight over-pressure to minimize ingress of external atmosphere. This immediate response ensures that oxygen concentrations within the chamber remain stable even when transferring items directly into or out of the chamber.
https://player.vimeo.com/video/1034221918?h=1ee1593366&app_id=122963&VIDEO: Effects of operating the easy-entry hatch under severe hypoxia
See our dedicated article: “The HypoxyLab easy-entry system“
Contamination control
HypoxyLab includes a built-in UV source within the water reservoir and a user replaceable HEPA filter that continuously scrubs the chamber atmosphere, ensuring that cultures and media are protected from contamination.
Touchscreen information display
Chamber oxygen, CO2, humidity and temperature are all set and controlled from the integrated touchscreen, which simultaneously displays the current real-time levels of these parameters in both digital and trace formats.
Cable thru-ports built in
In support of powered devices deployed within the HypoxyLab (digital microscopes, shakers, micro-centrifuges, etc.) the HypoxyLab features two dedicated thru-ports for the transfer of power and/or data cables up to 7 mm in diameter through the chamber wall without compromising the internal environment. The HypoxyLab ships with a pair of blanking glands.
The optional thru-port gland kit is described here.
Waste / vacuum thru-port built in
A 6 mm stainless steel pass-thru waste port is also built into the chamber wall as standard, intended for the aspiration of media, supernatants etc. from within the chamber via an externally connected vacuum pump.
Data logging
Internal oxygen, CO2, temperature and humidity parameters are continuously recorded onto internal flash memory and can be exported to a USB flash drive. Data files can subsequently be analysed and played back using the free LabChart® Reader by ADInstruments.
Cost of ownership advantage
HypoxyLab’s compact design and advanced gas control algorithms ensure rapid and ultra-smooth transitions to setpoints, with industry-leading gas consumption performance. Together with service-free sensors and a user changeable HEPA filter, these characteristics minimize the long term cost of ownership.
2-year product warranty
Our confidence in the reliability of the HypoxyLab is reflected in our comprehensive manufacturer’s warranty, covering defects in materials and workmanship for a period of 2 years from the date of delivery. Extended warranty packages, tied into preventative maintenance servicing are also available.
Unmatched technical support
Technical and application support is both comprehensive and free throughout the lifetime of our products. It is provided by highly experienced support staff who pride themselves in providing personal and responsive assistance via on-site visits, video calls, or email and will ‘go the extra mile’ to ensure that our users can extract the very best value and opportunity from our products.